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Cytotoxicity of PEGylated graphene oxide on lymphoma cells

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Du, Li | Wu, Shaoling; | Li, Yanhui; | Zhao, Xindong | Ju, Xiaoyan | Wang, Yuzhen

Affiliations: Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, 16 Jiangsu Road, Qingdao, Shandong 266003, China | Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, 308 Ningxia Road, Qingdao, Shandong 266071, China

Note: [] Corresponding authors: Shaoling Wu, Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, 16 Jiangsu Road, Qingdao, Shandong 266003, China. Tel.: +86 532 82911393; E-mail: qdwushaoling@163.com.

Note: [] Yanhui Li, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, 308 Ningxia Road, Qingdao, Shandong, 266071, China. Tel.: +86 532 85951842; E-mail: liyanhui@tsinghua.org.cn.

Keywords: PEGylated graphene oxide, lymphoma cells, cytotoxicity, CCK-8

DOI: 10.3233/BME-141024

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 2135-2141, 2014

Published: 2014
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Abstract

Graphene oxide (GO) is a hotspot, especially in the field of biomedical. However, the clinical application of GO is still faces a lot of challenges. In order to improve the solubility and biocompatibility of GO, polyethylene glycol (PEG) was grafted on the surface of graphene oxide by amide reaction. PEGylated graphene oxide (PEG-GO) was characterized using Fourier transform infrared spectroscopy (FTIR). The stability of PEG-GO detected in different solutions. Raji cell was selected as a lymphoma cell model to study the cytotoxicity of PEG-GO. Cell viability was detected using the Cell Counting Kit-8 assay. Cells were treated with different concentrations (10–100 μg/mL) of PEG-GO at different time points (6, 12, and 24 h). The FTIR spectrum of PEG-GO indicated that polyethylene glycol was successfully grafted onto GO. PEG-GO had excellent stability in all solutions. Cells treated with PEG-GO (10–100 μg/mL) for 24 hours had survival rates were over 80%. These results demonstrate that PEG-GO had an excellent dispersion in biological solutions and the toxicity of PEG-GO to lymphoma cells was low. The paper may provide cytological evidence for the application of PEG-GO in medicine.

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