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Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Zhao, Wenfang | Liu, Kehai; | Chen, Shunsheng | Zhu, Man man | Lv, Hui | Hu, Jing | Mao, Yuan

Affiliations: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Note: [] Corresponding author: Kehai Liu, Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. Tel.: +086-21-61900388; Fax: +086-21-61900365; E-mail: khliu@shou.edu.cn.

Keywords: Non-viral gene vector, polyethylenimine, P123, nuclear localization signal, avß3

DOI: 10.3233/BME-141002

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 1933-1939, 2014

Published: 2014
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Abstract

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100–250nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

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