Affiliations: Environment and Water Directorate, Ministry of Science and Technology, Baghdad, Iraq | [a] Al-Nahrain Center for Strategic Studies, Baghdad, Iraq | [b] Plant Protection Directorate, Ministry of Agriculture, Baghdad, Iraq
Abstract: Bioremediation of pesticides is the best option available to date due to its eco-friendly, cost-effective and efficacious nature. The study aimed to evaluate the Azotobacter spp. bioremediation Chemosate in the different incubation period and concentrations (5, 10, 15, 20, 25 ppm). From local sites, different microbes were isolated and Azotobacter separated using selective methods for identification of characteristics. The best result for the growth of Azotobacter sp. was at 25 ppm/0.222-0.163, in 15 days; in addition, the great degradation rate % was 25 ppm / 54.16%, observed in 2 months, while the best degradation and residues of chemosate after its digestion through MSM and HPLC residues analyses were at 25 ppm, as seen in 1-2 months, respectively. The degradation ratio % reached 81-79 % for 1-2 months. This conclusion suggests that Azotobacter spp. degradation Chemosate principles applied via hydrolysis binds phosphorus bonds with oxygen and digests the pesticides to produce nitrogen and carbon as elements for its growth sequences, especially at 2 months/25ppm.
