Affiliations: Hutchinson Technology Inc., IJsselburcht 3, Arnhem, The Netherlands | Tissue Engineering, Medical Biology, Department of Pathology Lab Medicine, Faculty of Medical Sciences, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands | Department of Medical Physiology, University of Groningen, A. Deusinglaan 1, 9713 AV, Groningen, The Netherlands
Abstract: Application of a Hyaff11-based nerve guide was studied in rats. Functional tests were performed to study motor nerve recovery. A withdrawal reflex test was performed to test sensory recovery. Morphology was studied by means of histology on explanted tissue samples. Motor nerve recovery was established within 7 weeks. Hereafter, some behavioral parameters like alternating steps showed an increase in occurence, while others remained stable. Sensory function was observed within the 7 weeks time frame. Nerve tissue had bridged the 10-mm gap within 7 weeks. The average nerve fiber surface area increased significantly in time. In situ degradation of the nerve conduit was fully going on at week 7 and tubes had collapsed by then. At weeks 15 and 21, the knitted tube wall structure was completely surrounded by macrophages and giant cells, and matrix was penetrating the tube wall. We conclude that a Hyaff11-based nerve guide can be used to bridge short peripheral nerve defects in rat. However, adaptations need to be made.
Keywords: Nerve repair, Hyaff11, rat sciatic nerve, morphology, immunohistology, macrophages
