Affiliations: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec H3A 2B4, Canada
Note: [] Corresponding Author: Dr S. Prakash, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec H3A 2B4, Canada. Tel: +1 514 398 3676, Fax: +1 514 298 3671, Email: satya.prakash@mcgill.ca
Abstract: Bile acids are important to normal human physiology. However, bile acids can be toxic when produced in pathologically high concentrations in hepatobileary and other diseases. This study shows that immobilized genetically engineered Lactobacillus plantarum 80 (pCBH1) (LP80 (pCBH1)) can efficiently hydrolyze bile acids and establishes a basis for their use. Results show that immobilized LP80 (pCBH1) is able to effectively break down the conjugated bile acids into glycodeoxycholic acid (GDCA) and taurodeoxycholic acid (TDCA) with bile salt hydrolase (BSH) activities of 0.17 and 0.07 μmol DCA/mg CDW/h, respectively. The deconjugation product, deoxycholic acid (DCA), was diminished by LP80 (pCBH1) within 4 h of initial BSH activity. This in-vitro study suggests that immobilized genetically engineered bacterial cells have important potential for deconjugation of bile acids for lowering of high levels of bile acids for therapy.
Keywords: Immobilization, bile acid, bile salt hydrolase, glycodeoxycholic acid, taurodeoxycholic acid, genetically engineered Lactobacillus plantarum cells
