Cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity

Article type: Research Article

Authors: Struski, Stéphanie | Doco‐Fenzy, Martine | Koehler, Michael | Chudoba, Ilse | Levy, Francis | Masson, Linda | Michel, Nicole | Ulrich, Evelyne | Gruson, Nadine | Bénard, Jean | Potron, Gérard | Cornillet‐Lefebvre, Pascale^;

Affiliations: Laboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20‐70‐IFR 53 Biomolecules), 51100 Reims, France | Laboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, France | ASI GmbH, 68309 Mannheim, Germany | MetaSystems, 68804 Altlussheim, Germany | Polytec‐PI, Electro‐optics Division, 93694 Pantin, France | Gustave Roussy Institute, 94800 Villejuif, France

Note: [] Corresponding author. Pascale Cornillet‐Lefebvre, Laboratoire d'Hématologie, Hôpital Robert Debré ‐ CHU Reims, 51092 Reims cedex, France. Tel.: +333 267 87789; Fax: +333 267 88171; E‐mail: plefebvre@chu‐reims.fr.

Abstract: In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex‐FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug‐resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐3/struski.htm.

Journal: Analytical Cellular Pathology, vol. 25, no. 3, pp. 115-122, 2003