Affiliations: Department of Surgery, University of Heidelberg, Heidelberg, Germany | Institute of Pathology, Charité Hospital, Humboldt‐University Berlin, Germany
Note: [] Corresponding author: Lukas Günther, M.D., Immunobiology Research Center, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Ave., #370, Boston, MA 02215, USA.
Abstract: A new staining method for dual demonstration of Estrogen receptors (ER) and argyrophilc Nucleolus‐Organizer Regions (AgNORs) was developed. To rule out possible reciprocal effects, serial slides of 10 invasive ductale breast cancers were stained with either the single staining method or the simultaneous ER/AgNOR‐staining method and investigated comparatively. By measuring the slides with the image analysis system AMBA, reciprocal effects could be excluded. It was proven that dual staining of both markers results in a reproducible and specific staining result. We concluded that it is justified to measure AgNORs in immunohistochemically stained cells.
Keywords: AgNORs, estrogen receptors, immunohistochemistry, breast cancer, dual staining, reproducibility
