Journal of Berry Research - Volume Pre-press, issue Pre-press
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Impact Factor 2019: 2.379
The main objective of the
Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems.
The objective will be achieved by focusing on four main areas of research and development:
1. From genetics to variety evaluation
2. Nursery production systems and plant quality control
3. Plant physiology, biochemistry and molecular biology, as well as cultural management
4. Health for the consumer: components and factors affecting berries' nutritional value
Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.
Journal of Berry Research will feature reviews, research articles, brief communications, position papers, letters and patent updates.
Abstract: BACKGROUND: Lower risk of digestive tract cancer development has been associated with polyphenol intake. Bactris guineensis is an edible endemic palm that grows in Central and South America. OBJECTIVE: This study performs a phenolic characterization of Bactris guineensis and evaluates the bioactivity of this fruit. METHODS: The phenolic compounds of B. guineensis were characterized by HPLC-UV-HRMS analyses and the antioxidant activity was measured by chemical and cellular methods. Additionally, cytotoxicity of B. guineensis polyphenols was performed on 4 cancer cell lines and the pro-apoptotic effect was evaluated by flow cytometry using annexin…staining. RESULTS: The major phenolic compounds of B. guineensis were proanthocyanidins. The extract IC50 for DPPH was 3.3±0.2μ g/mL and for induced intracellular ROS was 153±13μ g/mL. MTT cytotoxic assays demonstrate IC50 values between 16.6 and 24.9μ g/mL for the colon and hepatic adenocarcinomas, with high selectivity effects towards cancer cells compared to non-tumor cells. A 20 to 50% early apoptotic effect was observed in cancer cells lines by Annexin/PI staining. CONCLUSIONS: B. guineensis evidenced an important radical scavenging activity and a strong cytotoxic activity against hepatic and colorectal carcinoma cells, showing better values than procyanidin extracts from other fruits previously described.
Abstract: BACKGROUND: It is known that 1-MCP delays softening in kiwifruit. Time to ripen (eating-window) and its variability are considered useful for planning commercial strategies. However, few studies report the firmness and quality changes during long-term cold storage. OBJECTIVES: To study the effects on ripening and sensory attributes of different 1-MCP treatments during long (180 d) and very long-term (≥210 d) cold storage. Then, the optimal 1-MCP dose was applied to determine the time to ripen after cold storage for 30 to 240 d. METHODS: Kiwifruits were treated with 1-MCP: 0.0 (control), 0.5 and 1.0μ L L–1…. Maturity indices and sensory analysis were achieved after 180 and 210 d of storage at 0 °C. Ethylene production was also measured. Firmness and number of fruits at eating-ripe state (at 20 °C) were monthly determined after 30 to 240 d of cold storage. RESULTS: 1-MCP (1.0μ L L–1 ) delayed kiwifruit softening and prolonged the storage to up 180 d. Longer periods were associated to losses due to over-ripe. Eating windows of 1-MCP-treated fruits were longer than those for untreated fruits. CONCLUSIONS: 1.0μ L L–1 1-MCP extends the postharvest life of ‘Hayward’ kiwifruit and prolongs the eating window, allowing to plan different commercialization strategies.
Keywords: softening, quality, 1-methylcyclopropene, very long-term cold storage
Abstract: BACKGROUND: The new approach of using UV-C irradiation followed by a specific dark period to control plant diseases has the potential to become a mainstream treatment in the production of strawberries and other fruits and vegetables. Thus, it is imperative to study the effects of this treatment on fruit quality. METHODS: In this study, short-day ‘Chandler’ strawberry plants grown in growth chamber from bloom to harvest were irradiated twice a week with nighttime UV-C light (253 nm peak value 12.36 J/m2 and the total dose of 50 J/m2 in the range of 240 –260 nm). The effects on…the content of key phenolic compounds in mature fruit were studied with targeted analysis and a non-targeted metabolomic approach based on ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS). Analysis of variance-principal component analysis (ANOVA-PCA) was used to associate variance with different experimental factors. RESULTS: Results indicate that the content of anthocyanins, glucosides and glucuronides of quercetin and kaempferol, catechin, pelargonidin rutinoside, and ellagic acid was not affected by UV-C treatment. ANOVA-PCA analysis of the metabolomic dataset showed significant differences on composition and content of strawberry metabolites between UV-C and control groups according to harvest time. CONCLUSIONS: Our findings strongly indicate that UV-C treatment of 50 J/m2 twice per week did not affect nutritional values of strawberry fruit. The metabolomic approach combined with ANOVA-PCA used to evaluate strawberry fruit quality after UV-C irradiation proved to be a very powerful tool in providing an overall insight into fruit quality and accurately determines the influence of each experimental factor.
Abstract: BACKGROUND: Berries are worldwide recognized as “superfoods” due to the high content of bioactive compounds and the health benefits deriving from their consumption. OBJECTIVE: The present study was planned to assess and to compare the chemical profile and the in vitro antioxidant, hypoglycaemic, and anti-inflammatory activities of Vaccinium corymbosum L. berries and leaves extracts obtained by different extraction procedures. Ethanol was chosen as solvent because it is a GRAS (Generally Recognized As Safe) and widely used for the extraction of polar compounds. METHODS: Different extraction techniques such asmaceration, ultrasound-assisted extraction, Soxhlet extractor and decoction,…have been applied by using food grade ethanol/water as solvent mixture, selected as environmentally friendly solvents. Extracts obtained from fruits and leaves were chemically investigated by liquid chromatography-electrospray ionization quadrupole-time of flight-mass spectrometry (LC-ESI-QTOF-MS) and for their potential in vitro antioxidant, hypoglycaemic, and anti-inflammatory effects. RESULTS: Some iridoids were detected for the first time in V. corymbosum . Dried leaves extracted by decoction and ethanol with Soxhlet apparatus showed the highest 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals scavenging activity with IC50 value of 0.77μg/mL, which is 2.2-time lower than that positive control ascorbic acid. A promising inhibition of the production of nitrate/nitrite, critical mediators of inflammation, was found. The decoction of berries showed the highest activity in counteracting nitric oxide (NO.) production. Furthermore, two leaves extracts (decoction of dried leaves and hydroalcoholic maceration of fresh leaves) were particularly active as α-amylase inhibitors with IC50 values of 16.16 and 20.55 μg/mL, respectively. CONCLUSIONS: This work could provide valuable basis for future research on V. corymbosum to improve recovery of specific active compounds such as flavonoids and iridoids
Keywords: Vaccinium corymbosum, extraction procedures, chemical profiles, antioxidant, anti-inflammatory
Abstract: BACKGROUND: Berry processing residues called pomaces are rich in polyphenols, sugars, organic acids, and minerals. Polyphenols are reported to reduce the risk of non-communicable diseases, including cardiovascular diseases, cancer, and diabetes mellitus, owing to their anti-inflammatory activity. OBJECTIVE: We aimed to assess the anti-inflammatory properties of five Vaccinium spp . berry pomace extracts using LPS-stimulated THP-1 monocytes and a COX-2 inhibition assay. METHODS: THP-1 monocytes were pre-incubated with chemically characterized bilberry, blueberry, American cranberry, bog cranberry, and lingonberry pomace extracts following LPS stimulation. NF-κ B nuclear translocation was assessed by flow cytometry. TNF-α , MMP-9, IL-23,…IL-10, IL-1β , CCL22, and IL-8 secretion was analyzed in cell culture supernatant by using Luminex assay and ELISA. The expression of IL-6 , TNF- α , IL-10, IL-23 , and TGF- β 1 was assessed by qPCR, and COX-2 activity was determined by a fluorometric inhibition assay. RESULTS: All berry pomace extracts tested, except for lingonberry, inhibited LPS-induced NF-κ B nuclear translocation. All five extracts significantly downregulated the expression of pro-inflammatory cytokines and inhibited COX-2 activity in vitro . CONCLUSIONS: Vaccinium spp . pomace extracts demonstrated anti-inflammatory properties in an LPS-stimulated THP-1 cell inflammation model and inhibited COX-2 activity. Thus, Vaccinium spp. berry pomace extracts could serve as a valuable source of anti-inflammatory compounds.
Abstract: BACKGROUND: The pathway of anthocyanin biosynthesis, and its alterations leading to berry colour modification, are well known in grape skin. This variability could affect both quantity and quality of pigment accumulation. OBJECTIVE: The present work is focused on 15 grapevine cultivars selected to represent a high variability in the phenotypical colour traits in order to highlight new polymorphisms related to the flavonoid pathway. METHODS: Twenty-one genes involved in the biosynthetic pathway of anthocyanins were studied via targeted resequencing and were correlated with phenotypic data (anthocyanin profiles and spectroscopy indices). RESULTS: Single nucleotide polymorphism…(SNP) and InDel (insertion/deletion) polymorphisms were detected. Out of 1751 polymorphic loci, 68% were SNPs and 32% were InDels (568). Cluster analysis and SPLS-DA were used to investigate the genetic relationships among the cultivars, confirming the large range of phenotypical variability. Statistically significant correlations were detected between accumulation of 3’ anthocyanins and genetic polymorphisms in two structural genes and one transcription factor putatively involved in the anthocyanin biosynthetic pathway. CONCLUSIONS: The understanding of the polymorphisms related to the anthocyanin accumulation could support future selection of new pink table grape varieties with increased appeal on the consumers.
Keywords: Colour, flavonoid, grape, InDel, pink berry, polymorphisms, SNP
Abstract: BACKGROUND: There is concern that DNA damage may occur in skin cells due to UV irradiation. In this study, we investigated whether extracts from grape skin, which should be discarded, can suppress DNA damage caused by UV radiation OBJECTIVE: This study aims to investigate the effectivity of GSE in diminishing UV-induced cytotoxicity in normal human epidermal keratinocytes (NHEK) cells. METHODS: The polyphenol content in GSE was carried out using the HPLC instrument. UV dose was selected by measurement of cell viability, and ELISA results of DNA photoproduct. Optimum extracts condition of grape skin selected by DNA…photoproduct content as well as at this condition; Bax/Bcl-2 ratio and cytochrome c gene expressions were evaluated by Western blotting. RESULTS: In this study, we confirmed that GSE protect against DNA damage-induced cell death. Condition for 80% EtOH for 24 h at 60 °C was suitable to extract for grape skin. Red grape skin (Zweigelt) was more effective than white grape skin (Niagara) in preventing (before irradiation) and repairing (after irradiation). UV-induced upregulation of Bax/Bcl-2 ratio and cytochrome c expression were reduced by GSE treatment. CONCLUSIONS: The study demonstrated a promising potential of GSEs in skin therapeutics application.
Abstract: BACKGROUND: Pomegranate juice (PJ) contains strong antioxidant polyphenols, and cardiovascular beneficial effects have been reported for the fruit. OBJECTIVE: Using a Langendorff’s model, we hypothesized that PJ has antiarrhythmic effects on isolated rat hearts. METHODS: The hearts in the control group (n = 10) were perfused with Krebs solution. The test groups (n = 10, each) were further perfused with PJ, L-NAME (L), or both (PJL). Following 30 min stabilization, all hearts experienced 30 min global ischemia and 120 min reperfusion. The hearts were monitored for the occurrence of single and salvo arrhythmias, ventricular tachycardia (VT), and ventricular fibrillation (VF). The…cardiac release of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and nitrite were measured. In addition, the markers of oxidative stress including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and malondialdehyde (MDA) were assayed in the homogenates of the hearts. RESULTS: The PJ group showed a significant reduction in the occurrence of arrhythmias, an improvement in redox markers, and a rise in nitrite release, compared to the control. Simultaneous treatment with L-NAME significantly reduced, but not fully abolished, these effects. CONCLUSIONS: The current study suggests strong anti-arrhythmic effects for PJ, which are mainly mediated by nitric oxide.
Abstract: BACKGROUND: High anthocyanin content and the presence of other bioactive compounds are attractive characteristics of strawberry fruits for healthy consumption. OBJECTIVES: To characterize the anthocyanin content and the presence of other bioactive compounds, including anthocyanin (total and predominant types) andantioxidant activity; and to determine the physico-chemical fruit quality parameters of two new strawberry cultivars. METHOD: Fruits of two new hybrids were extracted and total anthocyanin and antioxidant activity were determined usinga UV-Vis spectrophotometer. Individual anthocyanins and vitamin C were measured using an HPLC. Physico-chemical characteristics of fruits were analyzed. RESULTS: Hybrid No. 4 line 5…and hybrid No. 4 line 26 are two potential new strawberry cultivars that are rich in anthocyanins. The total anthocyanin contents of these two hybrids were approximately 31–38 mg/100 g FW with no significant differences between them. Cyanidin 3-glucoside and pelargonidin 3-glucoside were foundat amounts of approximately 15–24 mg/kgFW and 332–478 mg/kg FW, respectively. Total phenolic compounds and FRAP activity of the two hybrids were approximately 2295–2579 mg GAE/kgFWand 27–30 mmol Fe2 + /kg FW, respectively. CONCLUSION: The two new hybrid strawberry lines, hybrid No. 4 line 5 and No. 4 line 26, when compared to the parents, had higher levels of bioactive compounds, especially anthocyanins, total phenolics, and FRAP, together with improved physico-chemical quality, and higher vitamin C content. These results indicate a considerable potential of these hybrids for commercial cultivation in Thailand and other production regions.